• Bioprospecting medicinal plants with biological potential
   

• Preparation of extracts for biological assays;

• Fractionation of extracts forbiological assays;

• Chemical characterization of the substance(s) responsible for the biological activity.
  
  
   

• Method development and validation by HPLC
   

• Development and validation of method for analysis of botanical extracts and phytomedicines.

• Optimization of chromatographic conditions based on the following parameters: column type, solvent type, injection volume, analysis time, temperature, elution type, mobile phase pH, buffer selection, among others.

• Validation of the chromatographic method for quantification of markers based on figures of merit: specificity/selectivity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness, in accordance with RDC RESOLUTION No. 166, OF JULY 24, 2017 .

• REFERENCES:

• HTTPS://WWW.IN.GOV.BR/MATERIA/-/ASSET_PUBLISHER/KUJRW0TZC2MB/CONTENT/ID/19194581/DO1-2017-07-25-RESOLUCAO-RDC-N-166-DE-24-DE-JULHO- DE-2017-19194412

• HTTP://STATIC.SITES.SBQ.ORG.BR/QUIMICANOVA.SBQ.ORG.BR/PDF/VOL27NO5_771_16-RV03165.PDF
  
  
   

• Development of a protocol for the preparation of ashows
   

• Technique used: multifactorial model as design of experiments (Design of Experiments - DOE).
  
  
   

• Development of environmentally friendly analytical processes
   

• Development of extraction and analysis protocols according to the 12 principles of Green Chemistry and use of green metrics to evaluate process performance.

• Ideal for companies looking to replace routine protocols with a high environmental impact, for new protocols with environmentally friendly processes.
  
  
   

• Scaling of analytical methods for the preparative scale
   

• Item Description
  
  
   

• Isolation of substances from plant matrices
   

• Technique used: liquid chromatography and medium pressure liquid chromatography on a preparative scale.

• Ability to isolate compounds with purity≥ 90%.
  
  
   

• Identification and characterization of molecules
   

• Technique used: HPLC-DAD-MS, high resolution mass spectrometry (HRMS) and nuclear magnetic resonance.
  
  
   

• Metabolomic analysis of plants
   

• Design and planning of experiments for target and untargetted metabolomics analysis.

• Sample analysis by nuclear magnetic resonance and LC-DAD-MS.

• Analysis of data generated through multivariate and univariate analysis.

• Identification of target substances through the use of databases.

• Development of protocols for isolation of the target substance.
  
  
   

• Essential oil analysis
   

• Technique used: gas chromatography coupled to mass spectrometry (GC-MS).

• The Kovats retention index (RI) and an internal database are used to identify the substances present in the essential oil.

• The IR is obtained from a standard mixture of hydrocarbons (C9-C24).

• It is possible to determine which substances are present in the essential oil (qualitative analysis), as well as the content of each one in the sample (quantitative analysis).
  
  
   

• Determination of fatty acids
   

• Technique used: gas chromatography coupled to mass spectrometry (GC-MS) and/or nuclear magnetic resonance (NMR).

• It is possible to determine which are the saturated and unsaturated fatty acids (qualitative analysis), as well as the content of each one in the vegetable or oil sample (quantitative analysis).

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• Isoflavon determinationto the
   

• ​Technique used: high performance liquid chromatography coupled to a diode array detector (HPLC-DAD).

• Analytical standard of the isoflavone (purity ≥ 98%) of interest is used for quantification.

• Examples of isoflavones: daidzein, genistein, daidzin, genistin, among others.

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• Determination of carotenoids
   

• Technique used: high performance liquid chromatography coupled to a diode array detector (HPLC-DAD).

• An analytical standard of the carotenoids (purity ≥ 98%) of interest is used for quantification.

• Examples of carotenoids: beta-carotene, bixin, lycopene, among others.

 

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• Determination of the content of total phenolic compounds
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• Determination in equivalents of gallic acid, chlorogenic acid, quercetin, among others.

• Analytical standard (purity ≥ 98%) is used for quantification.

• Technique used: spectrophotometry
  
  
   

• Determination of ta contentkids
   

• Technique used: spectrophotometry

• Determination of total tannins; condensed and/or hydrolyzable.

• Analytical standard (purity ≥ 98%) is used for quantification.